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Capping protein-controlled actin polymerization shapes lipid membranes.
Dürre, Katharina; Keber, Felix C; Bleicher, Philip; Brauns, Fridtjof; Cyron, Christian J; Faix, Jan; Bausch, Andreas R.
Afiliação
  • Dürre K; Lehrstuhl für Zellbiophysik, Technische Universität München, James-Franck-Str. 1, D-85748, Garching, Germany.
  • Keber FC; Lehrstuhl für Zellbiophysik, Technische Universität München, James-Franck-Str. 1, D-85748, Garching, Germany.
  • Bleicher P; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08544, USA.
  • Brauns F; Lehrstuhl für Zellbiophysik, Technische Universität München, James-Franck-Str. 1, D-85748, Garching, Germany.
  • Cyron CJ; Arnold Sommerfeld Center for Theoretical Physics and Center for NanoScience, Department of Physics, Ludwig-Maximilians-Universität München, Theresienstraße 37, D-80333, München, Germany.
  • Faix J; Lehrstuhl für Numerische Mechanik, Technische Universität München, Boltzmannstraße 15, D-85748, Garching, Germany.
  • Bausch AR; Institut für Biophysikalische Chemie, Medizinische Hochschule Hannover, Carl-Neuberg Straße 1, D-30625, Hannover, Germany.
Nat Commun ; 9(1): 1630, 2018 04 24.
Article em En | MEDLINE | ID: mdl-29691404
ABSTRACT
Arp2/3 complex-mediated actin assembly at cell membranes drives the formation of protrusions or endocytic vesicles. To identify the mechanism by which different membrane deformations can be achieved, we reconstitute the basic membrane deformation modes of inward and outward bending in a confined geometry by encapsulating a minimal set of cytoskeletal proteins into giant unilamellar vesicles. Formation of membrane protrusions is favoured at low capping protein (CP) concentrations, whereas the formation of negatively bent domains is promoted at high CP concentrations. Addition of non-muscle myosin II results in full fission events in the vesicle system. The different deformation modes are rationalized by simulations of the underlying transient nature of the reaction kinetics. The relevance of the regulatory mechanism is supported by CP overexpression in mouse melanoma B16-F1 cells and therefore demonstrates the importance of the quantitative understanding of microscopic kinetic balances to address the diverse functionality of the cytoskeleton.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Actinas / Proteínas de Capeamento de Actina Limite: Animals Idioma: En Revista: Nat Commun Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Actinas / Proteínas de Capeamento de Actina Limite: Animals Idioma: En Revista: Nat Commun Ano de publicação: 2018 Tipo de documento: Article