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Loss-of-function mutation in Hippo suppressed enlargement of lysosomes and neurodegeneration caused by dFIG4 knockdown.
Kushimura, Yukie; Azuma, Yumiko; Mizuta, Ikuko; Muraoka, Yuuka; Kyotani, Akane; Yoshida, Hideki; Tokuda, Takahiko; Mizuno, Toshiki; Yamaguchi, Masamitsu.
Afiliação
  • Kushimura Y; Department of Neurology.
  • Azuma Y; Department of Neurology.
  • Mizuta I; Department of Neurology.
  • Muraoka Y; Department of Applied Biology.
  • Kyotani A; The Center for Advanced Insect Research, Kyoto Institute of Technology, Kyoto, Japan.
  • Yoshida H; Department of Applied Biology.
  • Tokuda T; The Center for Advanced Insect Research, Kyoto Institute of Technology, Kyoto, Japan.
  • Mizuno T; Department of Applied Biology.
  • Yamaguchi M; The Center for Advanced Insect Research, Kyoto Institute of Technology, Kyoto, Japan.
Neuroreport ; 29(10): 856-862, 2018 07 04.
Article em En | MEDLINE | ID: mdl-29742619
ABSTRACT
Charcot-Marie-Tooth disease (CMT) is the most common hereditary neuropathy, and more than 80 CMT-causing genes have been identified to date. CMT4J is caused by a loss-of-function mutation in the Factor-Induced-Gene 4 (FIG4) gene, the product of which plays important roles in endosome-lysosome homeostasis. We hypothesized that Mammalian sterile 20-like kinase (MST) 1 and 2, tumor-suppressor genes, are candidate modifiers of CMT4J. We therefore examined the interaction between dFIG4 and Hippo (hpo), Drosophila counterparts of FIG4 and MSTs, respectively, using the Drosophila CMT4J model with the knockdown of dFIG4. The loss-of-function allele of hpo improved the rough eye morphology, locomotive dysfunction accompanied by structural defects in the presynaptic terminals of motoneurons, and the enlargement of lysosomes caused by the knockdown of dFIG4. Therefore, we identified hpo as a modifier of phenotypes induced by the knockdown of dFIG4. These results in Drosophila may provide an insight into the pathogenesis of CMT4J and contribute toward the development of disease-modifying therapy for CMT. We also identified the regulation of endosome-lysosome homeostasis as a novel probable function of Hippo/MST.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Charcot-Marie-Tooth / Proteínas Serina-Treonina Quinases / Proteínas de Drosophila / Peptídeos e Proteínas de Sinalização Intracelular / Lisossomos / Mutação Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neuroreport Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doença de Charcot-Marie-Tooth / Proteínas Serina-Treonina Quinases / Proteínas de Drosophila / Peptídeos e Proteínas de Sinalização Intracelular / Lisossomos / Mutação Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neuroreport Ano de publicação: 2018 Tipo de documento: Article