KIF20A expression as a prognostic indicator and its possible involvement in the proliferation of ovarian clearcell carcinoma cells.
Oncol Rep
; 40(1): 195-205, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29749467
ABSTRACT
Kinesin family member 20A (KIF20A), which is involved in cytokinesis and intracellular transportation, has been recently reported to be upregulated in several malignancies and may contribute to chemotherapeutic resistance. We examined the distribution and expression of KIF20A in clearcell carcinoma (CCC) of the ovary to elucidate its clinical significance and molecular mechanism. Paraffin sections from ovarian CCC tissues (N=43) were immunostained with KIF20A antibody, and the staining intensities were semiquantitatively evaluated. Furthermore, we investigated whether silencing of KIF20A contributes to the proliferationinhibitory potential using CCC cells. During the observational period, 18 patients (41.9%) developed recurrence. The median time to recurrence was 11.5 months. Patients in the high KIF20A expression group showed poorer progressionfree survival (PFS) and overall survival (OS) than those in the low expression group (P=0.0443 and P=0.0478, respectively). In multivariable analyses, KIF20A expression was also a significantly independent indicator of PFS and a marginally significant indicator of OS [PFS HR (high vs. low), 5.488; 95% CI, 1.41024.772 (P=0.0136); OS HR, 2.835; 95% CI, 0.85411.035, (P=0.0897)]. In in vitro studies, the ovarian CCC cell proliferation was significantly decreased by KIF20A silencing or in the presence of KIF20A inhibitor in CCC cells. Cell cycle G2/M arrest and a higher apoptosisinduced fraction were more frequently observed in siKIF20Atransfected CCC cells than in the control cells. Although the present study was preliminary, these data indicate the possible involvement of KIF20A in the proliferation of CCC, suggesting that targeting this molecule may contribute to reversing the malignant potential consequently affecting the oncologic outcome of CCC patients.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
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Prognóstico
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Biomarcadores Tumorais
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Cinesinas
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Adenocarcinoma de Células Claras
Tipo de estudo:
Prognostic_studies
Limite:
Aged
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Oncol Rep
Ano de publicação:
2018
Tipo de documento:
Article