Generation of GTKO Diannan Miniature Pig Expressing Human Complementary Regulator Proteins hCD55 and hCD59 via T2A Peptide-Based Bicistronic Vectors and SCNT.

Liu, Fengjuan; Liu, Jinji; Yuan, Zaimei; Qing, Yubo; Li, Honghui; Xu, Kaixiang; Zhu, Wanyun; Zhao, Heng; Jia, Baoyu; Pan, Weirong; Guo, Jianxiong; Zhang, Xuezeng; Cheng, Wenmin; Wang, Wei; Zhao, Hong-Ye; Wei, Hong-Jiang

Liu, Fengjuan; Liu, Jinji; Yuan, Zaimei; Qing, Yubo; Li, Honghui; Xu, Kaixiang; Zhu, Wanyun; Zhao, Heng; Jia, Baoyu; Pan, Weirong; Guo, Jianxiong; Zhang, Xuezeng; Cheng, Wenmin; Wang, Wei; Zhao, Hong-Ye; Wei, Hong-Jiang.

Afiliação

Liu F; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Liu J; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Yuan Z; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Qing Y; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Li H; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Xu K; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Zhu W; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Zhao H; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Jia B; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Pan W; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Guo J; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Zhang X; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Cheng W; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Wang W; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Zhao HY; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Wei HJ; College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.

Mol Biotechnol ; 60(8): 550-562, 2018 Aug.

Article em En | MEDLINE | ID: mdl-29916131

Assuntos

Antígenos CD55/genética; Antígenos CD59/genética; Galactosiltransferases/deficiência; Vetores Genéticos/genética; Técnicas de Transferência Nuclear; Peptídeos/genética; Animais; Animais Geneticamente Modificados; Antígenos CD55/imunologia; Antígenos CD55/metabolismo; Antígenos CD59/imunologia; Antígenos CD59/metabolismo; Ensaio de Atividade Hemolítica de Complemento; Feminino; Fibroblastos/metabolismo; Galactosiltransferases/metabolismo; Expressão Gênica; Humanos; Peptídeos/química; Gravidez; RNA Mensageiro/metabolismo; Suínos; Porco Miniatura/genética; Distribuição Tecidual; Transplante Heterólogo

Palavras-chave

GGTA1-knockout; Human complement regulatory proteins; T2A-based bicistronic vector; Xenotransplantation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Antígenos CD59 / Antígenos CD55 / Técnicas de Transferência Nuclear / Galactosiltransferases / Vetores Genéticos Limite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Mol Biotechnol Ano de publicação: 2018 Tipo de documento: Article

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