First evaluation of endotoxins in veterinary autogenous vaccines produced in Italy by LAL assay.
Biologicals
; 55: 71-73, 2018 Sep.
Article
em En
| MEDLINE
| ID: mdl-29935930
ABSTRACT
Endotoxin contamination is a serious concern for manufacturers of biological products and vaccines in terms of not only quality but also safety parameters. We evaluated the endotoxin presence in different veterinary autogenous vaccines produced by the Pharmaceutical Unit at the Experimental Zooprophylactic Institute of Umbria and Marche "Togo Rosati" (IZSUM). According to the 3Rs principles (Replace, Reduce, Refine), which aim to progressively reduce animal use in the quality control process, we tested the vaccines obtained from gram-negative bacteria and adjuvants by the limulus amebocyte lysate (LAL) assay. The results revealed low endotoxin concentrations compared to available data in the literature and represent the first report of the application of the 3Rs principles to veterinary autogenous vaccines production in Italy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotoxinas
/
Autovacinas
/
Teste do Limulus
Tipo de estudo:
Evaluation_studies
Limite:
Animals
País/Região como assunto:
Europa
Idioma:
En
Revista:
Biologicals
Ano de publicação:
2018
Tipo de documento:
Article