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Sucrose preference test for measurement of stress-induced anhedonia in mice.
Liu, Meng-Ying; Yin, Chun-Yu; Zhu, Li-Juan; Zhu, Xian-Hui; Xu, Chu; Luo, Chun-Xia; Chen, Hongshan; Zhu, Dong-Ya; Zhou, Qi-Gang.
Afiliação
  • Liu MY; Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
  • Yin CY; Department of Pharmacy, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
  • Zhu LJ; Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
  • Zhu XH; Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
  • Xu C; Key Laboratory of Developmental Genes and Human Diseases, MOE, Department of Histology and Embryology, School of Medicine, Southeast University, Nanjing, China.
  • Luo CX; Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
  • Chen H; Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
  • Zhu DY; Department of Clinical Pharmacology, Pharmacy College, Nanjing Medical University, Nanjing, China.
  • Zhou QG; Key Laboratory of Cardiovascular & Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, Nanjing, China. hongshanchen@njmu.edu.cn.
Nat Protoc ; 13(7): 1686-1698, 2018 07.
Article em En | MEDLINE | ID: mdl-29988104
Anhedonia is the inability to experience pleasure from rewarding or enjoyable activities and is a core symptom of depression in humans. Here, we describe a protocol for the measurement of anhedonia in mice, in which anhedonia is measured by a sucrose preference test (SPT) based on a two-bottle choice paradigm. A reduction in the sucrose preference ratio in experimental relative to control mice is indicative of anhedonia. To date, inconsistent and variable results have been reported following the use of the SPT by different groups, probably due to the use of different protocols and equipment. In this protocol, we describe how to set up a clearly defined apparatus for SPT and provide a detailed protocol to ensure greater consistency when carrying out SPT. This optimized protocol is highly sensitive, reliable, and adaptable for evaluation of chronic stress-related anhedonia, as well as morphine-induced dependence. The whole SPT, including adaptation, baseline measurement, and testing, takes 8 d.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estresse Psicológico / Sacarose / Edulcorantes / Testes Diagnósticos de Rotina / Anedonia / Preferências Alimentares Tipo de estudo: Prognostic_studies Aspecto: Patient_preference Limite: Animals Idioma: En Revista: Nat Protoc Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estresse Psicológico / Sacarose / Edulcorantes / Testes Diagnósticos de Rotina / Anedonia / Preferências Alimentares Tipo de estudo: Prognostic_studies Aspecto: Patient_preference Limite: Animals Idioma: En Revista: Nat Protoc Ano de publicação: 2018 Tipo de documento: Article