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Cloning and expression of the lectin gene from the mushroom Agrocybe aegerita and the activities of recombinant lectin in the resistance of shrimp white spot syndrome virus infection.
Chang, Yun-Shiang; Chen, Jian-Nan; Chang, Kun-Hung; Chang, Yi-Ming; Lai, Ying-Jang; Liu, Wang-Jing.
Afiliação
  • Chang YS; Department of Molecular Biotechnology, Da-Yeh University, Changhua, Taiwan.
  • Chen JN; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
  • Chang KH; Department of Molecular Biotechnology, Da-Yeh University, Changhua, Taiwan.
  • Chang YM; Department of Earth and Life Science, University of Taipei, Taipei, Taiwan.
  • Lai YJ; Department of Food Science, National Quemoy University, Kinmen, Taiwan.
  • Liu WJ; Department of Earth and Life Science, University of Taipei, Taipei, Taiwan. Electronic address: liuwjing@utaipei.edu.tw.
Dev Comp Immunol ; 90: 1-9, 2019 01.
Article em En | MEDLINE | ID: mdl-30031870
Lectin is a protein with multiple functions. In this study, the full-length cDNA of the Agrocybe aegerita lectin (AAL) gene was cloned, recombinant AAL (AAL-His) was expressed, and the activities of AAL-His were analyzed. Northern blot analysis showed that the major AAL transcript is approximately 900 bp. Sequence analysis showed that the coding region of AAL is 489 bp with a transcription start site located 39 nucleotides upstream of the translation initiation codon. In an agglutination test, AAL-His agglutinated rabbit erythrocytes at 12.5 µg/ml. AAL-His also showed antiviral activity in protecting shrimp from white spot syndrome virus (WSSV) infection. This anti-WSSV effect might be due to the binding of AAL-His on WSSV virions via the direct interactions with four WSSV structural proteins, VP39B, VP41B, VP53A and VP216. AAL demonstrates the potential for development as an anti-WSSV agent for shrimp culture. It also implies that these four AAL interaction WSSV proteins may play important roles in virus infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transgenes / Penaeidae / Vírus da Síndrome da Mancha Branca 1 / Infecções por Vírus de DNA / Agrocybe / Lectinas / Antígenos de Fungos Limite: Animals Idioma: En Revista: Dev Comp Immunol Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transgenes / Penaeidae / Vírus da Síndrome da Mancha Branca 1 / Infecções por Vírus de DNA / Agrocybe / Lectinas / Antígenos de Fungos Limite: Animals Idioma: En Revista: Dev Comp Immunol Ano de publicação: 2019 Tipo de documento: Article