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Genetic dissection of clonal lineage relationships with hydroxytamoxifen liposomes.
Ransom, Ryan C; Foster, Deshka S; Salhotra, Ankit; Jones, Ruth Ellen; Marshall, Clement D; Leavitt, Tripp; Murphy, Matthew P; Moore, Alessandra L; Blackshear, Charles P; Brett, Elizabeth A; Wan, Derrick C; Longaker, Michael T.
Afiliação
  • Ransom RC; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Foster DS; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Salhotra A; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Jones RE; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Marshall CD; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Leavitt T; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Murphy MP; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Moore AL; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Blackshear CP; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Brett EA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Wan DC; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Longaker MT; Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Nat Commun ; 9(1): 2971, 2018 07 30.
Article em En | MEDLINE | ID: mdl-30061668
ABSTRACT
Targeted genetic dissection of tissues to identify precise cell populations has vast biological and therapeutic applications. Here we develop an approach, through the packaging and delivery of 4-hydroxytamoxifen liposomes (LiTMX), that enables localized induction of CreERT2 recombinase in mice. Our method permits precise, in vivo, tissue-specific clonal analysis with both spatial and temporal control. This technology is effective using mice with both specific and ubiquitous Cre drivers in a variety of tissue types, under conditions of homeostasis and post-injury repair, and is highly efficient for lineage tracing and genetic analysis. This methodology is directly and immediately applicable to the developmental biology, stem cell biology and regenerative medicine, and cancer biology fields.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tamoxifeno / Linhagem da Célula / Lipossomos Limite: Animals Idioma: En Revista: Nat Commun Ano de publicação: 2018 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tamoxifeno / Linhagem da Célula / Lipossomos Limite: Animals Idioma: En Revista: Nat Commun Ano de publicação: 2018 Tipo de documento: Article