Investigation of the Applicability of a Rapid Diagnosis Test in the Diagnosis of Cystic Echinococcosis
Turkiye Parazitol Derg
; 42(2): 118-121, 2018 06.
Article
em En
| MEDLINE
| ID: mdl-30070642
ABSTRACT
OBJECTIVE:
Echinococcus granulosus, the etiological agent of cystic echinococcosis (CE) in humans and livestock, is a widely distributed zoonotic pathogen tapeworm. The infection is transmitted to humans by the ingestion of E. granulosus eggs released in the feces of definitive hosts such as dogs. The larval stage of the parasite develops a slowly enlarging cyst in the visceral organs, particularly in the liver and/or lung. The aim of the present study was to evaluate the diagnostic value of an immunochromatographic test (ICT) for CE.METHODS:
A total of 50 sera from surgically and/or pathologically confirmed patients with CE were included in the study as the study group; the control group comprised patients who tested negative for enzyme-linked immunosorbent assay (ELISA). Sera were selected from the collection at Adnan Menderes University, Faculty of Medicine, Parasitology Laboratory, by simple random sampling. The collection included sera obtained between 2010 and 2014; antibody titers of each serum sample were determined using in-house ELISA, before storage at -20°C. The presence of E. granulosus antibody in the sera was determined using a commercially available ICT (VIRAPID® HYDATIDOSIS) kit method.RESULTS:
In the study group (E. granulosus-confirmed cases), two (4%) of the 50 sera were negative and 48 (96%) were positive with ICT. In the control group (ELISA-negative), all were negative with ICT.CONCLUSION:
The rapid diagnostic test has been evaluated as a practical, easy-to-use method for detecting CE, and it can be used as a screening test in routine diagnosis and research.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Diagnóstico Pré-Natal
/
Complicações Parasitárias na Gravidez
/
Equinococose
/
Equinococose Hepática
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Observational_studies
Limite:
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Middle aged
/
Pregnancy
País/Região como assunto:
Asia
Idioma:
En
Revista:
Turkiye Parazitol Derg
Ano de publicação:
2018
Tipo de documento:
Article