ADPr-Peptide Synthesis.
Methods Mol Biol
; 1813: 345-369, 2018.
Article
em En
| MEDLINE
| ID: mdl-30097880
ABSTRACT
Synthetic mono-ADPr-peptides are useful for structural, biochemical, and proteomics studies. We describe here a protocol for the preparation of mono-ADPr-peptides based on a fairly standard Fmoc-based solid-phase synthesis. Phosphoribosylated precursor building blocks are introduced into the peptide chain on solid-phase and subsequently converted to ADPr-sites by chemical phosphorylation with adenosine phosphoramidite. Suitably protected phosphoribosylated glutamine, asparagine, and citrulline building blocks described in this protocol allow introduction of ADP-Gln, ADPr-Asn, and ADPr-Cit into peptide chains as demonstrated for three peptides. Trifunctional amino acids, for which base-sensitive side-chain protection is available, can be accommodated in the sequences flanking the ADPr-cites.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese Peptídica
/
Adenosina Difosfato Ribose
/
Técnicas de Síntese em Fase Sólida
/
ADP-Ribosilação
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2018
Tipo de documento:
Article