Electrical discharges in water induce spores' DNA damage.
PLoS One
; 13(8): e0201448, 2018.
Article
em En
| MEDLINE
| ID: mdl-30102709
ABSTRACT
Bacterial spores are one of the most resilient life forms on earth and are involved in many human diseases, such as infectious diarrhea, fatal paralytic illnesses and respiratory infections. Here, we investigated the mechanisms involved in the death of Bacillus pumilus spores after exposure to electric arcs in water. Cutting-edge microscopies at the nanoscale did not reveal any structural disorganization of spores exposed to electric arcs. This result suggested the absence of physical destruction by a propagating shock wave or an exposure to an electric field. However, Pulsed-Field Gel Electrophoresis (PFGE) revealed genomic DNA damage induced by UV radiation and Reactive Oxygen Species (ROS). UV induced single-strand DNA breaks and thymine dimers while ROS were mainly involved in base excision. Our findings revealed a correlation between DNA damage and the treatment of spores with electrical discharges.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
1_ASSA2030
/
2_ODS3
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3_ND
/
4_TD
Base de dados:
MEDLINE
Assunto principal:
Esporos Bacterianos
/
Dano ao DNA
/
DNA Bacteriano
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Purificação da Água
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Eletricidade
Limite:
Humans
Idioma:
En
Revista:
PLoS One
Ano de publicação:
2018
Tipo de documento:
Article