Terminal uridylyltransferases target RNA viruses as part of the innate immune system.
Nat Struct Mol Biol
; 25(9): 778-786, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-30104661
ABSTRACT
RNA viruses are a major threat to animals and plants. RNA interference (RNAi) and the interferon response provide innate antiviral defense against RNA viruses. Here, we performed a large-scale screen using Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV), and we identified cde-1 as important for antiviral defense. CDE-1 is a homolog of the mammalian TUT4 and TUT7 terminal uridylyltransferases (collectively called TUT4(7)); its catalytic activity is required for its antiviral function. CDE-1 uridylates the 3' end of the OrV RNA genome and promotes its degradation in a manner independent of the RNAi pathway. Likewise, TUT4(7) enzymes uridylate influenza A virus (IAV) mRNAs in mammalian cells. Deletion of TUT4(7) leads to increased IAV mRNA and protein levels. Collectively, these data implicate 3'-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Nucleotidiltransferases
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Vírus de RNA
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Caenorhabditis elegans
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Imunidade Inata
Limite:
Animals
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Humans
Idioma:
En
Revista:
Nat Struct Mol Biol
Ano de publicação:
2018
Tipo de documento:
Article