CRISPR-SKIP: programmable gene splicing with single base editors.
Genome Biol
; 19(1): 107, 2018 08 15.
Article
em En
| MEDLINE
| ID: mdl-30107853
ABSTRACT
CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with increased precision, such as single-base editors, are preferred. We present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pareamento de Bases
/
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
/
Edição de Genes
Limite:
Humans
Idioma:
En
Revista:
Genome Biol
Ano de publicação:
2018
Tipo de documento:
Article