Simultaneous determination of PUFA-derived pro-resolving metabolites and pathway markers using chiral chromatography and tandem mass spectrometry.
Anal Chim Acta
; 1031: 185-194, 2018 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-30119738
ABSTRACT
Lipid mediators play an important role as biological messengers involved in inflammatory processes. Deriving from different polyunsaturated fatty acids, endogenously built mediators featuring both pro- and anti-inflammatory properties as well as pro-resolving lipid mediators and their biological precursors have been investigated. A newly developed method using chiral chromatography-tandem mass spectrometry on human plasma has demonstrated its suitability for the simultaneous determination of prostaglandins, lipoxins, D-series derived resolvins as well as protectins, maresin 1, leukotriene B4 and several precursors of them in order to yield information about metabolic pathways. Due to the matrix complexity, a solid phase extraction method using an octadecyl-modified silica gel cartridge was carried out. The developed method allows the determination of 34 analytes in 25â¯min showing enough selectivity as well as precision and accuracy (≤15% relative standard deviation, ≤15% relative error) in the calibration range of 0.1-10â¯ngâ¯mL-1 or 0.2-20â¯ngâ¯mL-1 depending on the analytes. Stability of the analytes in plasma has been demonstrated for at least 3â¯hâ¯at room temperature, 72â¯h in the autosampler and 60 days in the freezer at -80⯰C. This method has been validated and shown its suitability for the determination of all studied analytes in human plasma samples.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Espectrometria de Massas em Tandem
/
Ácidos Graxos Insaturados
Limite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2018
Tipo de documento:
Article