[Frequency of CagA-positive Helicobacter pylori strains in 160 patients subjected to endoscopy]. / Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales.
Rev Med Chil
; 146(5): 596-602, 2018 May.
Article
em Es
| MEDLINE
| ID: mdl-30148923
ABSTRACT
BACKGROUND:
Helicobacter pylori is the most significant pathogen associated with gastric diseases, including gastric cancer. Infected patients with strains that are CagA-positive generally have worse outcomes than those infected with CagA-negative strains. Patients infected with CagA-positive strains have a higher risk for developing gastric cancer.AIM:
To determine the prevalence of CagA-positive H. pylori strains in fecal samples of patients from the Coquimbo Region of Chile, using a non-invasive, nested-qPCR method. MATERIAL ANDMETHODS:
We evaluated 160 patients with gastrointestinal symptoms subjected to an upper gastrointestinal endoscopy. DNA was extracted from fecal samples and tested for the presence of H. pylori using nested-qPCR for the ureC gene, and subsequently compared with the results of histology-Giemsa stain from the patients' endoscopic biopsies. When H. pylori was found, the presence of CagA-positive strains was determined via nested-qPCR.RESULTS:
The histology-Giemsa stain was positive for H. pylori infection in 123 patients (76.9%), while the analysis of fecal samples detected H. pylori in 129 patients (80.6%). The sensitivity and specificity of nested-qPCR to detect the bacterium was 96.7 and 73.0% respectively. Among patients with the infection, 25% had CagA-positive strains.CONCLUSIONS:
In this sample of patients, there is a low prevalence of CagA-positive H. pylori strains.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gastropatias
/
Proteínas de Bactérias
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DNA Bacteriano
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Helicobacter pylori
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Infecções por Helicobacter
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Fezes
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Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
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Prevalence_studies
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Risk_factors_studies
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Screening_studies
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
Es
Revista:
Rev Med Chil
Ano de publicação:
2018
Tipo de documento:
Article