FACS - based isolation of human eosinophils allows purification of high quality RNA.
J Immunol Methods
; 463: 47-53, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30217720
ABSTRACT
Here we extensively describe a FACS-based protocol for isolating intact non-stained human eosinophils from peripheral blood; a stop forward from our recently published initial study. This method of purification could be accomplished in <3â¯h with only small volumes of whole blood necessary, even in healthy subjects generally exhibiting low levels of circulating eosinophils. Eosinophil activation during the isolation steps appeared to be minimal and this purification procedure yielded high quality RNA. Moreover, these FACS-isolated eosinophils had prolonged viability in culture and were suitable for further activation assays.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
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Eosinófilos
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Citometria de Fluxo
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2018
Tipo de documento:
Article