Repeated sequences with unusual properties from the divergent region of Crithidia oncopelti maxicircle kinetoplast DNA.

ABSTRACT

The occurrence of bacterial promoter-like sequences was shown in the divergent region of Crithidia oncopelti maxicircular kinetoplast DNA. A promoter-cloning vector based on the neomycin phosphotransferase II (NPT II) gene was used to localize promoters in three homologous blocks of repeated sequences. The elements found displayed greater promoter strength in Escherichia coli than the normal promoter of the NPT II gene. Sequences of three promoter-containing inserts from different blocks were determined and typical prokaryotic promoter sequences were localized.