Your browser doesn't support javascript.
loading
Analysis of coding and non-coding transcriptome of peripheral B cells reveals an altered interferon response factor (IRF)-1 pathway in multiple sclerosis patients.
Annibali, Viviana; Umeton, Renato; Palermo, Antonia; Severa, Martina; Etna, Marilena Paola; Giglio, Simona; Romano, Silvia; Ferraldeschi, Michela; Buscarinu, Maria Chiara; Vecchione, Andrea; Annese, Anita; Policano, Claudia; Mechelli, Rosella; Pizzolato Umeton, Raffaella; Fornasiero, Arianna; Angelini, Daniela Francesca; Guerrera, Gisella; Battistini, Luca; Coccia, Eliana Marina; Salvetti, Marco; Ristori, Giovanni.
Afiliação
  • Annibali V; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Umeton R; Department of Informatics, Dana-Farber Cancer Institute, Boston, MA, United States; Massachusetts Institute of Technology, Cambridge, MA, United States.
  • Palermo A; Department of Mathematics and Computer Science, University of Calabria.
  • Severa M; Department of Infectious, Parasitic and Immune-mediated Disease, Istituto Superiore di Sanità, Rome, Italy.
  • Etna MP; Department of Infectious, Parasitic and Immune-mediated Disease, Istituto Superiore di Sanità, Rome, Italy.
  • Giglio S; Division of Pathology, Department of Clinical and Molecular Medicine, Sant'Andrea Hospital, Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Romano S; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Ferraldeschi M; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Buscarinu MC; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Vecchione A; Division of Pathology, Department of Clinical and Molecular Medicine, Sant'Andrea Hospital, Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Annese A; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Policano C; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Mechelli R; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Pizzolato Umeton R; Department of Neurology, University of Massachusetts Memorial Medical Center, Worcester, MA, USA.
  • Fornasiero A; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy.
  • Angelini DF; Neuroimmunology Unit, Fondazione Santa Lucia, Rome, Italy.
  • Guerrera G; Neuroimmunology Unit, Fondazione Santa Lucia, Rome, Italy.
  • Battistini L; Neuroimmunology Unit, Fondazione Santa Lucia, Rome, Italy.
  • Coccia EM; Department of Infectious, Parasitic and Immune-mediated Disease, Istituto Superiore di Sanità, Rome, Italy.
  • Salvetti M; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy; IRCCS Istituto Neurologico Mediterraneo (INM) Neuromed (M.S.), Pozzilli, IS, Ita
  • Ristori G; Center for Experimental Neurological Therapies, Sant'Andrea Hospital, Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Sapienza University of Rome, Rome, Italy. Electronic address: giovanni.ristori@uniroma1.it.
J Neuroimmunol ; 324: 165-171, 2018 11 15.
Article em En | MEDLINE | ID: mdl-30270021
ABSTRACT
Several evidences emphasize B-cell pathogenic roles in multiple sclerosis (MS). We performed transcriptome analyses on peripheral B cells from therapy-free patients and age/sex-matched controls. Down-regulation of two transcripts (interferon response factor 1-IRF1, and C-X-C motif chemokine 10-CXCL10), belonging to the same pathway, was validated by RT-PCR in 26 patients and 21 controls. IRF1 and CXCL10 transcripts share potential seeding sequences for hsa-miR-424, that resulted up-regulated in MS patients. We confirmed this interaction and its functional effect by transfection experiments. Consistent findings indicate down-regulation of IRF1/CXCL10 axis, that may plausibly contribute to a pro-survival status of B cells in MS.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos B / Transdução de Sinais / Perfilação da Expressão Gênica / Fator Regulador 1 de Interferon / Transcriptoma / Esclerose Múltipla Tipo de estudo: Diagnostic_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Neuroimmunol Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos B / Transdução de Sinais / Perfilação da Expressão Gênica / Fator Regulador 1 de Interferon / Transcriptoma / Esclerose Múltipla Tipo de estudo: Diagnostic_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Neuroimmunol Ano de publicação: 2018 Tipo de documento: Article