Low-Cost Method Generating In Situ Anaerobic Conditions on a 96-Well Plate for Microbial Fermentation in Food Research.
J Agric Food Chem
; 66(44): 11839-11845, 2018 Nov 07.
Article
em En
| MEDLINE
| ID: mdl-30277075
ABSTRACT
Commercial tools and instruments have been developed for a screening study of microbial fermentation, but they are expensive and mostly confined to aerobic fermentation only. There is little development on the generation of anaerobic conditions directly on a 96-well plate. This report proposed a simple and versatile microbial fermentation system known as OVAMO that makes use of Oxyrase, vacuum, and mineral oil to generate an in situ anaerobic environment on a 96-well plate for at least 48 h. The practicality of OVAMO in anaerobic fermentation experiments used for functional food research was validated by a prebiotic screening study of different carbohydrates by Bifidobacterium longum subsp. infantis. The OVAMO system provides a less expensive but effective way to conduct a microbial fermentation screening study that requires anaerobic conditions without the need for atmospheric control by external devices.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bifidobacterium
/
Microbiologia de Alimentos
Tipo de estudo:
Evaluation_studies
/
Health_economic_evaluation
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2018
Tipo de documento:
Article