Synthesis of Cytokinins via Enzymatic Arsenolysis of Purine Nucleosides.
Curr Protoc Nucleic Acid Chem
; 75(1): e61, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30299587
ABSTRACT
This unit describes an effective method for the preparation of natural cytokinins and their synthetic derivatives based on enzymatic cleavage of the N-glycosidic bond of N6 -substituted adenosine or O6 -substituted inosine derivatives in the presence of purine nucleoside phosphorylase (PNP) and Na2 HAsO4 . The arsenolysis reaction is irreversible due to the hydrolysis of the resulting α-D-ribose-1-arsenate. As a result, the desired products are formed in near-quantitative yields, as indicated by high-performance liquid chromatography (HPLC) analysis, and can easily be isolated. In the strategy used here, the ribose residue acts as a protective group. © 2018 by John Wiley & Sons, Inc.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arseniatos
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Nucleosídeos de Purina
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Purina-Núcleosídeo Fosforilase
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Citocininas
Idioma:
En
Revista:
Curr Protoc Nucleic Acid Chem
Ano de publicação:
2018
Tipo de documento:
Article