Analysis of Drugs in Oral Fluid Using LC-MS/MS.
Methods Mol Biol
; 1872: 237-259, 2019.
Article
em En
| MEDLINE
| ID: mdl-30350295
ABSTRACT
Oral fluid analysis for drugs is increasingly used in a variety of testing areas pain management and medication monitoring, parole and probation situations, driving under the influence of drugs (DUID), therapeutic drug monitoring, and testing for drugs in the workplace. The sample collection itself is straightforward, rapid, observable, and noninvasive, requiring no special facilities (compared to urine) or medical personnel (compared to blood). The pH of saliva is slightly acidic relative to blood; therefore, drugs which are more basic tend to be present in higher concentration in oral fluid than in blood cocaine, amphetamines, oxycodone, tramadol, buprenorphine, methadone, and fentanyl. Conversely, acidic drugs and drugs which are strongly protein bound have lower concentrations in oral fluid than in blood examples include benzodiazepines, barbiturates, and carisoprodol. Because of the low volume of specimen available for analysis and the drug concentrations present (generally much lower than those in urine), efficient extraction methods and sensitive confirmation procedures are necessary for routine analysis of drugs in oral fluid. In this chapter, solid-phase extraction methods are described for a variety of drugs with liquid chromatography-tandem mass spectrometry detection.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Detecção do Abuso de Substâncias
/
Cromatografia Líquida
/
Monitoramento de Medicamentos
/
Espectrometria de Massas em Tandem
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2019
Tipo de documento:
Article