Reaction mechanism of ß-apiosidase from Aspergillus aculeatus.
Food Chem
; 274: 543-546, 2019 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-30372976
ABSTRACT
Apiosidases are glycosidases relevant for aroma development during fermentation of wines and black tea. Reaction mechanism of apiosidase from Aspergillus aculeatus in commercial glycanase Viscozyme L was studied by 1H NMR technique. Study of hydrolysis of 4-nitrophenyl ß-D-apiofuranoside revealed that this reaction proceeds with inversion of hydroxyl group in the anomeric center, which confirms inverting mechanism of the enzyme and its inability to catalyze transapiosylation in syntheses of apiosides.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspergillus
/
Glucosidases
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2019
Tipo de documento:
Article