A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases.
Chem Commun (Camb)
; 54(95): 13411-13414, 2018 Nov 27.
Article
em En
| MEDLINE
| ID: mdl-30427336
ABSTRACT
Metal complexes can initiate DNA cleavage by the formation of reactive oxygen species (ROS). A conventional assay to probe for ROS is to add quenchers in a gel electrophoresis experiment. As we show here, such an assay is neither selective nor reliable. Instead, we suggest the use of simple fluorogens, as tested here with several metallonucleases for the detection of H2O2 and HOË.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radical Hidroxila
/
Complexos de Coordenação
/
Corantes Fluorescentes
/
Peróxido de Hidrogênio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Chem Commun (Camb)
Ano de publicação:
2018
Tipo de documento:
Article