Identification of novel circadian transcripts in the zebrafish retina.
J Exp Biol
; 222(Pt 1)2019 01 10.
Article
em En
| MEDLINE
| ID: mdl-30446534
ABSTRACT
High fecundity, transparent embryos for monitoring the rapid development of organs and the availability of a well-annotated genome has made zebrafish a model organism of choice for developmental biology and neurobiology. This vertebrate model, which is also a favourite in chronobiology studies, shows striking circadian rhythmicity in behaviour. Here, we identify novel genes in the zebrafish genome that are expressed in the zebrafish retina. We further resolve the expression pattern over time and tentatively assign specific novel transcripts to retinal bipolar cells of the inner nuclear layer. Using chemical ablation and free run experiments, we segregate the transcripts that are rhythmic when entrained by light from those that show sustained oscillations in the absence of external cues. The transcripts reported here with rigorous annotation and specific functions in circadian biology provide the groundwork for functional characterization of novel players in the zebrafish retinal clock.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Peixe-Zebra
/
Ritmo Circadiano
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Proteínas de Peixe-Zebra
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Exp Biol
Ano de publicação:
2019
Tipo de documento:
Article