Strategies for humanizing glycosylation pathways and producing recombinant glycoproteins in microbial expression systems.
Biotechnol Prog
; 35(2): e2752, 2019 03.
Article
em En
| MEDLINE
| ID: mdl-30457225
ABSTRACT
Homogeneously glycosylated proteins are essential for analyzing the structure of N-glycans, studying their consequences inside cells, and developing therapeutic glycoproteins. However, the isolation of glycoproteins with homogeneous glycans from human is difficult since glycoforms slightly differ from each other with respect to molecular weight and charge. Microbial expression systems have numerous benefits in expression technology and have gained great attention, because they are more adaptable to the biotechnology industry. While selecting an expression host, the glycosylation pattern must be taken into account, because glycosylation strongly depends on cellular production system and selected production clone. This review discussed the technological developments in glycoengineering of microbial expression systems for humanizing the glycosylation profile and highlighted the expression potential of Leishmania expression system. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35 e2752, 2019.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Proteínas Recombinantes
/
Glicoproteínas
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Escherichia coli
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Leishmania
Limite:
Humans
Idioma:
En
Revista:
Biotechnol Prog
Ano de publicação:
2019
Tipo de documento:
Article