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Single dose of 17ß-estradiol provides transient neuroprotection in female juvenile mice after cardiac-arrest and cardiopulmonary resuscitation.
Quillinan, N; Dingman, A L; Deng, G; Tatum, S; Orfila, J E; Clevenger, A C; Klawitter, J; Traystman, R J; Herson, P S.
Afiliação
  • Quillinan N; Department of Anesthesiology, University of Colorado, Anschutz Medical Campus, USA.
  • Dingman AL; Department of Pediatrics, Division of Child Neurology, Intensive Care Unit, University of Colorado, Anschutz Medical Campus, USA.
  • Deng G; Department of Pharmacology, University of Colorado, Anschutz Medical Campus, USA.
  • Tatum S; Department of Anesthesiology, University of Colorado, Anschutz Medical Campus, USA.
  • Orfila JE; Department of Anesthesiology, University of Colorado, Anschutz Medical Campus, USA.
  • Clevenger AC; Department of Pediatrics, Intensive Care Unit, University of Colorado, Anschutz Medical Campus, USA.
  • Klawitter J; Department of Anesthesiology, University of Colorado, Anschutz Medical Campus, USA.
  • Traystman RJ; Department of Anesthesiology, University of Colorado, Anschutz Medical Campus, USA.
  • Herson PS; Department of Anesthesiology, University of Colorado, Anschutz Medical Campus, USA; Department of Pharmacology, University of Colorado, Anschutz Medical Campus, USA. Electronic address: paco.herson@ucdenver.edu.
Neurochem Int ; 127: 80-86, 2019 07.
Article em En | MEDLINE | ID: mdl-30471325
Each year there are approximately 7000 out of hospital cardiac arrests in the pediatric population, with 30% resuscitation rate and a 6-10% rate of survival to hospital discharge. Survivors of cardiac arrest exhibit learning and memory deficits that are devastating during the school years. Delayed neuronal cell death occurs in the hippocampus following cardiac arrest and likely contributes to memory impairments. Circulating endogenous estrogen in young adult females has been shown to provide protection against ischemic cell death, as does chronic exogenous administration of 17ß-estradiol (E2). Chronic estrogen benefit can have undesirable feminizing effects, particularly in pre-adolescents. Here, we tested if a single-dose of E2 is neuroprotective in our pediatric cardiac arrest mouse model performed in juvenile mice. We subjected P21P25 C57Blk6 male and female mice to 8 min of cardiac arrest followed by cardiopulmonary resuscitation (CA/CPR). This developmental stage preceded the hormonal onset and serum estradiol and testosterone levels were not different in males and females. A single dose of E2 (100µg/kg) or vehicle was administered 30 min after resuscitation. Neuronal cell death measured 3 days after CA/CPR showed reduced hippocampal cell death in E2-treated females, but not males. Benefit of E2 in females was blocked by the P38 MAPK inhibitor, SB203580. Hippocampal-dependent memory function was equally impaired in E2-and vehicle-treated females measured in the contextual fear conditioning task at 7 days. Our findings demonstrate female-specific transient neuroprotection with E2 that does not provide sustained functional benefit.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 6_ODS3_enfermedades_notrasmisibles Base de dados: MEDLINE Assunto principal: Isquemia Encefálica / Estradiol / Neuroproteção / Parada Cardíaca Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neurochem Int Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 6_ODS3_enfermedades_notrasmisibles Base de dados: MEDLINE Assunto principal: Isquemia Encefálica / Estradiol / Neuroproteção / Parada Cardíaca Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neurochem Int Ano de publicação: 2019 Tipo de documento: Article