(+)pinoresinolOßDglucopyranoside from Eucommia ulmoides Oliver and its antiinflammatory and antiviral effects against influenza A (H1N1) virus infection.
Mol Med Rep
; 19(1): 563-572, 2019 01.
Article
em En
| MEDLINE
| ID: mdl-30483751
ABSTRACT
Eucommia ulmoides Oliver (Du-Zhong) is an ancient Chinese herbal remedy used for the treatment of various diseases. To date, the effects of its constituent lignans on influenza viruses remain to be elucidated. In the present study, a lignan glycoside was isolated and purified from Eucommia ulmoides Oliver. Its structures were identified via extensive spectroscopic analysis, and its antiviral and antiinflammatory activities, specifically against influenza viruses, were determined via a cytopathic effect (CPE) assay, plaquereduction assays, a progeny virus yield reduction assay, reverse transcriptionquantitative polymerase chain reaction analysis and a Luminex assay. Additionally, western blot analysis was performed to investigate the underlying mechanisms of its effects against influenza viruses. The chemical and spectroscopic methods determined the structure of lignan glycoside to be (+)pinoresinolOßDglucopyranoside. The CPE assay showed that (+)pinoresinolOßDglucopyranoside exerted inhibitory activities with 50% inhibition concentration values of 408.81±5.24 and 176.24±4.41 µg/ml against the influenza A/PR/8/34 (H1N1) and A/Guangzhou/GIRD07/09 (H1N1) strains, respectively. Its antiviral properties were confirmed by plaque reduction and progeny virus yield reduction assays. Additional mechanistic analyses indicated that the antiH1N1 virusinduced effects of (+)pinoresinolO-ßD-glucopyranoside were likely due to inactivation of the nuclear factorκB, p38 mitogenactivated protein kinase and AKT signaling pathways. Furthermore, (+)pinoresinolOßDglucopyranoside exhibited pronounced inhibitory effects on the expression of influenza H1N1 virusinduced proinflammatory mediators, including tumor necrosis factorα, interleukin (IL)6, IL8 and monocyte chemoattractant protein 1. The data obtained suggest that (+)pinoresinolOßD-glucopyranoside may be a candidate drug for treating influenza H1N1 virus infection.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Antivirais
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Lignanas
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Eucommiaceae
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Influenza Humana
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Vírus da Influenza A Subtipo H1N1
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Furanos
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Glicosídeos
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Anti-Inflamatórios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2019
Tipo de documento:
Article