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Harmonization of pipeline for preclinical multicenter plasma protein and miRNA biomarker discovery in a rat model of post-traumatic epileptogenesis.
Kamnaksh, Alaa; Puhakka, Noora; Ali, Idrish; Smith, Gregory; Aniceto, Roxanne; McCullough, Jesse; Das Gupta, Shalini; Ndode-Ekane, Xavier Ekolle; Brady, Rhys; Casillas-Espinosa, Pablo; Hudson, Matt; Santana-Gomez, Cesar; Immonen, Riikka; Abreu, Pedro Andrade de; Jones, Nigel; Shultz, Sandy; Staba, Richard J; O'Brien, Terence J; Agoston, Denes; Pitkänen, Asla.
Afiliação
  • Kamnaksh A; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Maryland, USA. Electronic address: alaa.kamnaksh.ctr@usuhs.edu.
  • Puhakka N; A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
  • Ali I; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Smith G; Department of Neurosurgery, David Geffen School of Medicine, University of California, Los Angeles, USA.
  • Aniceto R; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Maryland, USA.
  • McCullough J; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Maryland, USA.
  • Das Gupta S; A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
  • Ndode-Ekane XE; A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
  • Brady R; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Casillas-Espinosa P; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Hudson M; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Santana-Gomez C; Department of Neurology, David Geffen School of Medicine at the University of California, Los Angeles, USA.
  • Immonen R; A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
  • Abreu PA; A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
  • Jones N; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Shultz S; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Staba RJ; Department of Neurology, David Geffen School of Medicine at the University of California, Los Angeles, USA.
  • O'Brien TJ; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia; Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Australia.
  • Agoston D; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Maryland, USA.
  • Pitkänen A; A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
Epilepsy Res ; 149: 92-101, 2019 01.
Article em En | MEDLINE | ID: mdl-30553097
The Epilepsy Bioinformatics Study for Antiepileptogenic Therapy (EpiBioS4Rx) is an international, multicenter, multidisciplinary study aimed at preventing epileptogenesis (EpiBioS4Rx: https://epibios.loni.usc.edu/). One of the study's major objectives is the discovery of diagnostic, prognostic, and predictive plasma protein and microRNA (miRNA) biomarkers that are sensitive, specific, and translatable to the human condition. Epilepsy due to structural brain abnormalities, secondary to neurological insults such as traumatic brain injury (TBI), currently represents ∼50% of all epilepsy cases. In the preclinical EpiBioS4Rx study, TBI was induced in adult male Sprague Dawley rats using a standardized protocol for lateral fluid-percussion injury. Whole blood was collected from the tail vein at baseline and 2, 9 and 30 days post-injury and processed for plasma separation. Biomaterial properties, sample preparation and integrity, and choice of analysis platform can significantly impact measured marker levels and, in turn, interpretation with respect to injury and/or other variables. We present here the results of procedural harmonization for the first 320 rats included in the EpiBioS4Rx study study, from three international research centers, and preliminary proteomic and miRNA analyses. We also discuss experimental considerations for establishing rigorous quality controls with the goal of harmonizing operating procedures across study sites, and delivering high-quality specimens for preclinical biomarker discovery in a rat model of post-traumatic epilepsy (PTE).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Sanguíneas / Epilepsia Pós-Traumática / MicroRNAs / Homeostase Tipo de estudo: Guideline / Prognostic_studies Limite: Animals Idioma: En Revista: Epilepsy Res Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Sanguíneas / Epilepsia Pós-Traumática / MicroRNAs / Homeostase Tipo de estudo: Guideline / Prognostic_studies Limite: Animals Idioma: En Revista: Epilepsy Res Ano de publicação: 2019 Tipo de documento: Article