Zhongshania marina sp. nov., isolated from deep-sea water.

ABSTRACT

A Gram-stain-negative, aerobic, catalase-positive, oxidase-positive, motile by a single polar flagellum, rod-shaped strain, designated DSW25-10T, was isolated from the deep-sea water of the East Sea, Republic of Korea. Strain DSW25-10Tgrew at 4-35 °C (optimum, 20-35 °C), at pH 5.5-9.0 (optimum, pH 7.0) and in the presence of 0-6.0 % NaCl (optimum, 0.5-2 %), and could assimilate valerate, but not assimilate d-mannitol and 3-hydroxy-butyrate. Comparative 16S rRNA gene sequence analysis showed that strain DSW25-10T belongs to the genus Zhongshania in the family Spongiibacteraceae and is most closely related to Zhongshania guokunii ZS6-22T, Zhongshania borealis CL-AS9T, Zhongshania aliphaticivorans SM-2T and Zhongshani antarctica ZS5-23T with a similarities of 97.1, 97.0, 96.7 and 96.6 %, respectively. The G+C content of genomic DNA was 49.1 mol% and the major respiratory quinone was ubiquinone-8. Phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were identified as the major cellular polar lipids. The predominant cellular fatty acids in strain DSW25-10T were summed feature 3 (comprising C16  1ω7c and/or C16  1ω6c), summed feature 8 (comprising C18  1ω7c and/or C18  1ω6c), C16  0 and C17  1ω8c. The DNA-DNA relatedness values between strain DSW25-10T and related strains were clearly lower than 70 %. On the basis of evidence from a polyphasic analysis, strain DSW25-10T is proposed to represent a novel species, Zhongshania marina sp. nov. The type strain is DSW25-10T (=KCCM 43273T=JCM 17372T).