Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies.
Virol J
; 15(1): 192, 2018 12 27.
Article
em En
| MEDLINE
| ID: mdl-30587193
ABSTRACT
Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
/
4_TD
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Testes Sorológicos
/
Testes de Neutralização
/
Programas de Rastreamento
/
Zika virus
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Infecção por Zika virus
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Humans
País/Região como assunto:
Caribe
/
Martinica
Idioma:
En
Revista:
Virol J
Ano de publicação:
2018
Tipo de documento:
Article