Nitrate, but not nitrite, derived from nitrogen dioxide accumulates in Arabidopsis leaves following exposure to 15N-labeled nitrogen dioxide.

ABSTRACT

It is known that when plant leaves are exposed to exogenously applied nitrogen dioxide (NO2), nitrogen derived from NO2 is reduced to amino acid nitrogen. However, whether this is the sole metabolic fate of exogenously applied NO2 is unclear. In this study, Arabidopsis leaves were exposed to 4 ppm 15N-labeled NO2 for 4 h in light, followed by capillary ion analysis and elemental analysis-mass spectrometry with an elemental analyzer connected directly to a mass spectrometer. We found that leaf cells exposed to 15N-labeled NO2 accumulated a large amount of 15N-labeled nitrate. Neither 15N-labeled nitrite nor endogenous nitrite was present in exposed leaves. It is likely that exogenously applied NO2 is first converted to nitrite, and that nitrite is oxidized to nitrate in Arabidopsis leaf cells. The complete disappearance of nitrite derived from exogenously applied NO2 and endogenous nitrite supports this mechanism.