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The rapid and sensitive detection of edible bird's nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay.
Lee, Meng-Shiou; Huang, Jhong-Yong; Lien, Yi-Yang; Sheu, Shyang-Chwen.
Afiliação
  • Lee MS; China Medical University, Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, Taichung, 40402, Taiwan.
  • Huang JY; National Pingtung University of Science and Technology, Department of Food Science, Pingtung, 91201, Taiwan.
  • Lien YY; National Pingtung University of Science and Technology, Department of Veterinary Medicine, Pingtung, 91201, Taiwan.
  • Sheu SC; National Pingtung University of Science and Technology, Department of Food Science, Pingtung, 91201, Taiwan.
J Food Drug Anal ; 27(1): 154-163, 2019 01.
Article em En | MEDLINE | ID: mdl-30648568
Edible bird's nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers' interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. Moreover, the sensitivity of LAMP in authenticating EBN was 10-8 µg, it showed higher sensitivity than that of conventional PCR with 105 fold. When genomic DNAs extracted from boiled or steamed EBN samples were used as templates, LAMP for EBN detection was not affected and was reproducible after heat processing. In conclusion, the LAMP assay established herein could be applicable for authenticating EBN and for identifying commercial EBN products in herbal markets.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aves / Técnicas de Amplificação de Ácido Nucleico / Análise de Alimentos Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Animals Idioma: En Revista: J Food Drug Anal Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aves / Técnicas de Amplificação de Ácido Nucleico / Análise de Alimentos Tipo de estudo: Diagnostic_studies / Evaluation_studies Limite: Animals Idioma: En Revista: J Food Drug Anal Ano de publicação: 2019 Tipo de documento: Article