High-Parameter Single-Cell Analysis.
Annu Rev Anal Chem (Palo Alto Calif)
; 12(1): 411-430, 2019 06 12.
Article
em En
| MEDLINE
| ID: mdl-30699035
ABSTRACT
Thousands of transcripts and proteins confer function and discriminate cell types in the body. Using high-parameter technologies, we can now measure many of these markers at once, and multiple platforms are now capable of analysis on a cell-by-cell basis. Three high-parameter single-cell technologies have particular potential for discovering new biomarkers, revealing disease mechanisms, and increasing our fundamental understanding of cell biology. We review these three platforms (high-parameter flow cytometry, mass cytometry, and a new class of technologies called integrated molecular cytometry platforms) in this article. We describe the underlying hardware and instrumentation, the reagents involved, and the limitations and advantages of each platform. We also highlight the emerging field of high-parameter single-cell data analysis, providing an accessible overview of the data analysis process and choice of tools.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Célula Única
/
Citometria de Fluxo
Limite:
Humans
Idioma:
En
Revista:
Annu Rev Anal Chem (Palo Alto Calif)
Ano de publicação:
2019
Tipo de documento:
Article