Alterations of RNA Metabolism by Proteomic Analysis of Breast Cancer Cells Exposed to Marycin: A New Optically Active Porphyrin.
Curr Mol Pharmacol
; 12(2): 147-159, 2019.
Article
em En
| MEDLINE
| ID: mdl-30714537
ABSTRACT
OBJECTIVE:
Marycin is a porphyrin-type compound synthetically modified to spontaneously release fluorescence. This study is aimed at understanding possible mechanisms that could account for the antiproliferative effects observed in marycin. A proteomic approach was used to identify molecular effects. The proteome of proliferating MDA-MB-231 breast cancer cells was compared with that of marycin-treated cells.METHODS:
Label-free proteomic analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to reveal changes in protein expression and fluorescence microscopy and flow cytometry were used to detect subcellular organelle dysfunctions.RESULTS:
The bioinformatic analysis indicated an enhancement of the expression of proteins remodeling RNA splicing and more in general, of RNA metabolism. Marycin did not localize into the mitochondria and did not produce a dramatic increase of ROS levels in MDA-MB-231 cells. Marycin stained organelles probably peroxisomes.CONCLUSIONS:
The results could support the possibility that the peroxisomes are involved in cell response to marycin.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Porfirinas
/
RNA
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Regulação Neoplásica da Expressão Gênica
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Proteômica
/
Hematoporfirinas
Limite:
Female
/
Humans
Idioma:
En
Revista:
Curr Mol Pharmacol
Ano de publicação:
2019
Tipo de documento:
Article