Measles vector as a multigene delivery platform facilitating iPSC reprogramming.
Gene Ther
; 26(5): 151-164, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30718755
Induced pluripotent stem cells (iPSCs) provide a unique platform for individualized cell therapy approaches. Currently, episomal DNA, mRNA, and Sendai virus-based RNA reprogramming systems are widely used to generate iPSCs. However, they all rely on the use of multiple (three to six) components (vectors/plasmids/mRNAs) leading to the production of partially reprogrammed cells, reducing the efficiency of the systems. We produced a one-cycle measles virus (MV) vector by substituting the viral attachment protein gene with the green fluorescent protein (GFP) gene. Here, we present a highly efficient multi-transgene delivery system based on a vaccine strain of MV, a non-integrating RNA virus that has a long-standing safety record in humans. Introduction of the four reprogramming factors OCT4, SOX2, KLF4, and cMYC via a single, "one-cycle" MV vector efficiently reprogrammed human somatic cells into iPSCs, whereas MV vector genomes are rapidly eliminated in derived iPSCs. Our MV vector system offers a new reprogramming platform for genomic modification-free iPSCs amenable for clinical translation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Transferência de Genes
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Reprogramação Celular
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Células-Tronco Pluripotentes Induzidas
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Técnicas de Reprogramação Celular
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Vírus do Sarampo
Limite:
Animals
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Humans
Idioma:
En
Revista:
Gene Ther
Ano de publicação:
2019
Tipo de documento:
Article