"Solvent/non-solvent" treatment as a method for non-covalent immobilization of gelatin on the surface of poly(l-lactic acid) electrospun scaffolds.
Colloids Surf B Biointerfaces
; 177: 137-140, 2019 May 01.
Article
em En
| MEDLINE
| ID: mdl-30721789
ABSTRACT
In the present study, we report a simple and efficient method of gelatin immobilization on the surface of PLA electrospun fibers using pre-treatment with a mixture of toluene and ethanol allowing to form swelled surface layer followed by gelatin adsorption from its solution in PBS. Our results demonstrate that gelatin immobilization leads to a decrease in the water contact angle from 120° to 0°, enhances scaffold strength up to 50%, and doubles the number of adhered cells and their average area. We observed that the maximum amount of gelatin (0.07 ± 0.01 mg per cm3 of the scaffold) was immobilized during the first five minutes of exposure to the gelatin solution. Modified scaffolds demonstrated increased strength.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Solventes
/
Materiais Biocompatíveis
/
Gelatina
Limite:
Humans
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Ano de publicação:
2019
Tipo de documento:
Article