Establishment of Novel Cells Stably Secreting Various Human IL-18 Recombinant Proteins.
Curr Pharm Biotechnol
; 20(1): 47-55, 2019.
Article
em En
| MEDLINE
| ID: mdl-30727885
ABSTRACT
BACKGROUND:
The immunotherapies against cancer, autoinmmune diseases or infection are remarkable development. These days programmed cell death (PD)-1 antibody-induced immune checkpoint blockade or chimeric antigen receptor-T cells (CAR-T) have been shown to have eminent therapeutic effects on tumor development. We have focused on adoptive transfer with human gamma delta T cells for novel immunotherapies. Additionally, IL-18 is one of the cytokines that enhances cytokine secretion and cytotoxicity of human gamma delta T cells.METHOD:
Thus, we established novel cell lines stably expressing and secreting various types of human recombinant IL-18 proteins to their culture supernatants using episomal vector. We also differentiated primary cultured human gamma delta T cells from peripheral blood mononuclear leukocytes to validate biological activity of the IL-18 proteins using measuring IFN-γ by ELISA. RESULTS ANDCONCLUSION:
Finally, we demonstrated that the supernatant could activate human gamma delta T cells using monitoring interferon gamma in culture medium.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
/
Interleucina-18
/
Linfócitos Intraepiteliais
Limite:
Humans
Idioma:
En
Revista:
Curr Pharm Biotechnol
Ano de publicação:
2019
Tipo de documento:
Article