Live cell super resolution imaging by radial fluctuations using fluorogen binding tags.
Nanoscale
; 11(8): 3626-3632, 2019 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-30734810
ABSTRACT
Fluorescence-Activating and absorption-Shifting Tag (FAST) is a novel genetically encoded optical highlighter probe. Since the fluorescence of FAST originates from the stochastic and reversible diffusive association of a fluorogenic ligand, we investigate the application of FAST using Super-Resolution Radial Fluctuations (SRRF) to achieve routine imaging below the diffraction limit in a widefield epifluorescence microscope. We show that intensity fluctuation analysis like SRRF allows the imaging of FAST-tagged proteins with sub - 100 nm resolution in live cells. FAST co-labeled with conventional fluorophores enables real time multicolour 2D and 3D super-resolution imaging, indicating that FAST can be used for the observation of sub-diffraction limited structures in both living and fixed samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Fluorescência Verde
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Revista:
Nanoscale
Ano de publicação:
2019
Tipo de documento:
Article