Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms.
Int J Nanomedicine
; 14: 937-950, 2019.
Article
em En
| MEDLINE
| ID: mdl-30787608
ABSTRACT
BACKGROUND:
Skeletal muscle tissue engineering often involves the prefabrication of muscle tissues in vitro by differentiation and maturation of muscle precursor cells on a platform which provides an environment that facilitates the myogenic differentiation of the seeded cells.METHODS:
Poly lactic-co-glycolic acid (PLGA) 3D printed scaffolds, which simulate the highly complex structure of extracellular matrix (ECM), were fabricated by E-jet 3D printing in this study. The scaffolds were used as platforms, providing environment that aids in growth, differentiation and other properties of C2C12 myoblast cells.RESULTS:
The C2C12 myoblast cells grown on the PLGA 3D printed platforms had enhanced cell adhesion and proliferation. Moreover, the platforms were able to induce myogenic differentiation of the myoblast cells by promoting the formation of myotubes and up-regulating the expressions of myogenic genes (MyHC and MyOG).CONCLUSION:
The fabricated 3D printed platforms have excellent biocompatibility, thereby can potentially be used as functional cell culture platforms in skeletal tissue engineering and regeneration.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diferenciação Celular
/
Mioblastos
/
Alicerces Teciduais
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Hidrodinâmica
/
Impressão Tridimensional
Limite:
Animals
Idioma:
En
Revista:
Int J Nanomedicine
Ano de publicação:
2019
Tipo de documento:
Article