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Synergistic lethality between BRCA1 and H3K9me2 loss reflects satellite derepression.
Padeken, Jan; Zeller, Peter; Towbin, Benjamin; Katic, Iskra; Kalck, Veronique; Methot, Stephen P; Gasser, Susan M.
Afiliação
  • Padeken J; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
  • Zeller P; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
  • Towbin B; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
  • Katic I; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
  • Kalck V; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
  • Methot SP; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
  • Gasser SM; Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland.
Genes Dev ; 33(7-8): 436-451, 2019 04 01.
Article em En | MEDLINE | ID: mdl-30804228
Caenorhabditis elegans has two histone H3 Lys9 methyltransferases, MET-2 (SETDB1 homolog) and SET-25 (G9a/SUV39H1 related). In worms, we found simple repeat sequences primarily marked by H3K9me2, while transposable elements and silent tissue-specific genes bear H3K9me3. RNA sequencing (RNA-seq) in histone methyltransferase (HMT) mutants shows that MET-2-mediated H3K9me2 is necessary for satellite repeat repression, while SET-25 silences a subset of transposable elements and tissue-specific genes through H3K9me3. A genome-wide synthetic lethality screen showed that RNA processing, nuclear RNA degradation, the BRCA1/BARD1 complex, and factors mediating replication stress survival are necessary for germline viability in worms lacking MET-2 but not SET-25. Unlike set-25 mutants, met-2-null worms accumulated satellite repeat transcripts, which form RNA:DNA hybrids on repetitive sequences, additively with the loss of BRCA1 or BARD1. BRCA1/BARD1-mediated H2A ubiquitination and MET-2 deposited H3K9me2 on satellite repeats are partially interdependent, suggesting both that the loss of silencing generates BRCA-recruiting DNA damage and that BRCA1 recruitment by damage helps silence repeats. The artificial induction of MSAT1 transcripts can itself trigger damage-induced germline lethality in a wild-type background, arguing that the synthetic sterility upon BRCA1/BARD1 and H3K9me2 loss is directly linked to the DNA damage provoked by unscheduled satellite repeat transcription.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas / Caenorhabditis elegans / Regulação da Expressão Gênica no Desenvolvimento / Proteína BRCA1 / Proteínas de Caenorhabditis elegans Limite: Animals Idioma: En Revista: Genes Dev Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas / Caenorhabditis elegans / Regulação da Expressão Gênica no Desenvolvimento / Proteína BRCA1 / Proteínas de Caenorhabditis elegans Limite: Animals Idioma: En Revista: Genes Dev Ano de publicação: 2019 Tipo de documento: Article