Evaluation of a new multiplex assay for allergy diagnosis.
Clin Chim Acta
; 493: 73-78, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30822401
ABSTRACT
BACKGROUND:
Molecular allergy has significantly improved the quality of allergy diagnosis; however, the positioning of singleplex and multiplex assays in the diagnostic algorithm is still a matter of debate.METHODS:
Aim of the study was to test the analytical performance of the recently commercialized Allergy Explorer-ALEX® in a selected population (105 allergic patients and 15 negative controls), comparing it with the reference ImmunoCAP® method and with skin prick test (SPT).RESULTS:
Inter-assay qualitative comparison showed a substantial agreement between ALEX® and SPT (kâ¯=â¯0.64). A substantial agreement between ALEX® and ImmunoCAP® was shown on the detection of IgE to extracts (kâ¯=â¯0.64 for inhalants and kâ¯=â¯0.51 for food allergens), whereas a higher agreement was shown on detection of molecular components (kâ¯=â¯0.92 for inhalants and kâ¯=â¯0.72 for food allergens). Quantitative comparison showed a poor correlation between ALEX® and ImmunoCAP®.CONCLUSION:
The simultaneous detection of both extracts and molecular components with ALEX® assay can potentially overcome some of the major limitations of the multiplex assay currently in use. However, before using ALEX® as routine method, the analytical performance (in particular for extracts) needs to be further investigated on a larger scale.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes Cutâneos
/
Alérgenos
/
Hipersensibilidade Alimentar
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Qualitative_research
Limite:
Adolescent
/
Adult
/
Aged
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
Europa
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
2019
Tipo de documento:
Article