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Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions.
Coelho Graça, Didia; Hartmer, Ralf; Jabs, Wolfgang; Scherl, Alexander; Clerici, Lorella; Samii, Kaveh; Tsybin, Yury O; Hochstrasser, Denis; Lescuyer, Pierre.
Afiliação
  • Coelho Graça D; Clinical Proteomics and Chemistry Group, Faculty of Medicine, University of Geneva, Geneva, Switzerland. didia.coelhograca@hcuge.ch.
  • Hartmer R; Department of Genetic, Laboratory Medicine and Pathology, Geneva University Hospitals, Geneva, Switzerland. didia.coelhograca@hcuge.ch.
  • Jabs W; Bruker Daltonics, Bremen, Germany.
  • Scherl A; Beuth Hochschule für Technik Berlin, Berlin, Germany.
  • Clerici L; Clinical Proteomics and Chemistry Group, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
  • Samii K; Department of Genetic, Laboratory Medicine and Pathology, Geneva University Hospitals, Geneva, Switzerland.
  • Tsybin YO; Department of Genetic, Laboratory Medicine and Pathology, Geneva University Hospitals, Geneva, Switzerland.
  • Hochstrasser D; Department of Genetic, Laboratory Medicine and Pathology, Geneva University Hospitals, Geneva, Switzerland.
  • Lescuyer P; Division of Hematology, Geneva University Hospitals, Geneva, Switzerland.
Methods Mol Biol ; 1959: 173-183, 2019.
Article em En | MEDLINE | ID: mdl-30852823
ABSTRACT
Characterization of protein structure modifications is an important field in mass spectrometry (MS)-based proteomics. Here, we describe a process to quickly and reliably identify a mass change in a targeted protein sequence by top-down mass spectrometry (TD MS) using electron transfer dissociation (ETD). The step-by-step procedure describes how to develop a TD MS method for data acquisition as well as the data analysis process. The described TD MS workflow utilizes diagnostic ions to characterize an unknown sample in a few hours.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteômica / Espectrometria de Massas em Tandem / Íons Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteômica / Espectrometria de Massas em Tandem / Íons Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2019 Tipo de documento: Article