A Practical Guide to Genome Editing Using Targeted Nuclease Technologies.
Compr Physiol
; 9(2): 665-714, 2019 03 14.
Article
em En
| MEDLINE
| ID: mdl-30873595
ABSTRACT
Genome engineering using programmable nucleases is a rapidly evolving technique that enables precise genetic manipulations within complex genomes. Although this technology first surfaced with the creation of meganucleases, zinc finger nucleases, and transcription activator-like effector nucleases, CRISPR-Cas9 has been the most widely adopted platform because of its ease of use. This comprehensive review presents a basic overview of genome engineering and discusses the major technological advances in the field. In addition to nucleases, we discuss CRISPR-derived base editors and epigenetic modifiers. We also delve into practical applications of these tools, including creating custom-edited cell and animal models as well as performing genetic screens. Finally, we discuss the potential for therapeutic applications and ethical considerations related to employing this technology in humans. © 2019 American Physiological Society. Compr Physiol 9665-714, 2019.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Desoxirribonucleases
/
Edição de Genes
Aspecto:
Ethics
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Compr Physiol
Ano de publicação:
2019
Tipo de documento:
Article