On-site quantitative Hg2+ measurements based on selective and sensitive fluorescence biosensor and miniaturized smartphone fluorescence microscope.
Biosens Bioelectron
; 132: 238-247, 2019 May 01.
Article
em En
| MEDLINE
| ID: mdl-30878724
ABSTRACT
Mercury is a bio-accumulative and toxic pollutant causing severe damages to human health and environment. Since Hg2+ is the most stable form of mercury, selective and sensitive Hg2+ detection is required. Though classical approaches can realize accurate Hg2+ detection, the complicated instruments and the time-consuming operations inevitably limit their on-site applications. Here, we design a smart Hg2+ detection approach using the fluorescence biosensor, the smartphone fluorescence microscope and the smartphone application for Hg2+ on-site detection. Based on the thymine-Hg2+-thymine coordination chemistry, a selective and sensitive fluorescence biosensor is designed for capturing Hg2+ in aqueous solution; besides, a miniaturized smartphone fluorescence microscope for fluorescence signal collection and an image processing application for quantitative Hg2+ measurements are constructed. A highly specific detection of Hg2+ with a linear relation between 1â¯nM and 1⯵M with a limit of detection of 1â¯nM is obtained using the smart Hg2+ detection approach. Considering it can realize selective and sensitive quantitative Hg2+ measurements in high precision with simple operations and cost-effective system, it is believed the proposed smart Hg2+ detection approach owns great potentials in Hg2+ detection for routine uses at home and in the field.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
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Técnicas Biossensoriais
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Smartphone
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Mercúrio
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Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biosens Bioelectron
Ano de publicação:
2019
Tipo de documento:
Article