Assessment of Sperm PAWP Expression in Infertile Men.
Urol J
; 16(5): 488-494, 2019 10 21.
Article
em En
| MEDLINE
| ID: mdl-30882161
ABSTRACT
PURPOSE:
The aim of this study was to evaluate expression of Post-Acrosomal WW Binding Protein (PAWP) in infertile men with low and high fertilization post ICSI and also globozoospermic men. MATERIALS ANDMETHODS:
Semen samples were collected from 18 infertile men with previously failed or low fertili-zation (< 25%) post ICSI, 10 men with high fertilization (>50%) post ICSI, 15 globozoospermic men, and 21 fertile individuals. Then, expression of PAWP was assessed at RNA with quantitative Real Time PCR.RESULTS:
Relative expression of PAWP in sperm was significantly (P < .05) lower in infertile men with globozo-ospermia (41.5 ± 5.7) or low fertilization rate (43.3±10.4) compared to fertile (138.8 ± 17.3)or men with high fer-tilization (211.6 ± 75.6). In addition, a significant positive correlation (r = 0.628; P = .001) was observed between percentage of fertilization with the relative expression of PAWP.CONCLUSION:
Considering solid recent evidences regarding PLC? as the main sperm factor involved in oocyte acti-vation, therefore co-localization of PLC? with PAWP in perinuclear theca may account for the above observation and it is likely that PAWP may have other functions and/or it may assist PLC?.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Proteínas de Transporte
/
Proteínas de Plasma Seminal
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Infertilidade Masculina
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Urol J
Ano de publicação:
2019
Tipo de documento:
Article