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Enzymatic hydrolysis of corn crop residues with high solid loadings: New insights into the impact of bioextrusion on biomass deconstruction using carbohydrate-binding modules.
Gatt, Etienne; Khatri, Vinay; Bley, Julien; Barnabé, Simon; Vandenbossche, Virginie; Beauregard, Marc.
Afiliação
  • Gatt E; Laboratoire de Chimie Agro-industrielle, LCA, Université de Toulouse, INRA, Toulouse, France. Electronic address: etienne.gatt@ensiacet.fr.
  • Khatri V; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada; PROTEO, Université Laval, Québec G1V 4G2, Canada. Electronic address: vkhatri@ualberta.ca.
  • Bley J; Centre de recherche sur les matériaux lignocellulosiques, Université du Québec à Trois-Rivières, Canada; Innofibre, 3351 Boulevard des Forges, Québec G9A 5E6, Canada.
  • Barnabé S; Centre de recherche sur les matériaux lignocellulosiques, Université du Québec à Trois-Rivières, Canada.
  • Vandenbossche V; Laboratoire de Chimie Agro-industrielle, LCA, Université de Toulouse, INRA, Toulouse, France. Electronic address: virginie.vandenbossche@ensiacet.fr.
  • Beauregard M; Centre de recherche sur les matériaux lignocellulosiques, Université du Québec à Trois-Rivières, Canada; PROTEO, Université Laval, Québec G1V 4G2, Canada.
Bioresour Technol ; 282: 398-406, 2019 Jun.
Article em En | MEDLINE | ID: mdl-30884460
Lignocellulosic biomass is a sustainable source of renewable substrate to produce low carbon footprint energy and materials. Biomass conversion is usually performed in two steps: a biomass pretreatment for improving cellulose accessibility followed by enzymatic hydrolysis of cellulose. In this study we investigated the efficiency of a bioextrusion pretreatment (extrusion in the presence of cellulase enzyme) for production of reducing sugars from corn crop agricultural residues. Our results demonstrate that bioextrusion increased the reducing sugar conversion yield by at least 94% at high solid/liquid ratio (14%-40%). Monitoring biomass surface with carbohydrate-binding modules (FTCM-depletion assay) revealed that well known negative impact of high solid/liquid ratio on conversion yield is not due to the lack of exposed cellulose which was abundant under such conditions. Bioextrusion was found to be less efficient on alkaline pretreated biomass but being a mild and solvent limiting pretreatment, it might help to minimize the waste stream.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomassa / Zea mays Idioma: En Revista: Bioresour Technol Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomassa / Zea mays Idioma: En Revista: Bioresour Technol Ano de publicação: 2019 Tipo de documento: Article