Bistachybotrysins F-J, five new phenylspirodrimane dimers with a central cyclopentanone linkage from Stachybotrys chartarum.

Bistachybotrysins F-J (1-5), five new phenylspirodrimane dimers with a central cyclopentanone core were isolated from Stachybotrys chartarum CGMCC 3.5365. The structures of 1-5 were elucidated through extensive spectroscopic data analysis, including 1D/2D NMR, HR-MS, and ECD spectra. Compounds 3-5 displayed moderate cytotoxic activity against the cell lines HCT116, NCI-H460, and HepG2 with IC50 values ranging from 9.1 to 12.2 µM, making them promising as lead compounds for drug research and discovery.