Mass Cytometric Cell Cycle Analysis.
Methods Mol Biol
; 1989: 193-215, 2019.
Article
em En
| MEDLINE
| ID: mdl-31077107
ABSTRACT
The regulated proliferation of cells is a critical factor in tumor progression, antineoplastic therapies, immune system regulation, and the cellular developmental of multicellular organisms. While measurement of cell cycle state by fluorescent flow cytometry is well established, mass cytometry allows the cell cycle to be measured along with large numbers of other antigens enabling characterization of the complex interactions between the cell cycle and wide variety of cellular processes. This method describes the use of mass cytometry for the analysis of cell cycle state for cells from three different sources in vitro cultured cell lines, ex vivo human blood or bone marrow, and in vivo labeling of murine tissues. The method utilizes incorporation of 5-Iodo-2'-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), Cyclin B1, and phosphorylated Histone H3 (pHH3). These measurements can be integrated into a gating strategy that enables clear separation of all five phases of the cell cycle.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
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Coloração e Rotulagem
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Histonas
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Ciclo Celular
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Proteína do Retinoblastoma
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Ciclina B1
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Citometria de Fluxo
Limite:
Animals
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Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2019
Tipo de documento:
Article