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[Establishment and evaluation of an animal model of female pelvic nerve injury].
Dai, Y X; Zhang, G R; Lang, J H; Zhu, L.
Afiliação
  • Dai YX; General Gynecology Division, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy and Medical Sciences & Peking Union Medical College, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi ; 99(17): 1336-1339, 2019 May 07.
Article em Zh | MEDLINE | ID: mdl-31091583
Objective: To build a stable animal model simulating pelvic nerve injury in female pelvic floor dysfunction. Methods: A total of 55 10-week-old female SD rats weighing (220±15) g were randomly divided into 3 groups: 5 for normal group, 25 for sham operation group (SO), 25 for bilateral pudendal nerve block group (BPNB). Samples of rat anterior vaginal wall were obtained in 3 days, 1 week, 1 month and 3 months after the operation. The number of nerve fibers was counted per high power field under microscope, with UCHL immunohistochemical staining of nerve fibers. RNA was extracted and the expression of RNA related to nerve tissue was tested. Results: The numbers of nerve fibers had no significant difference between the normal group and the sham operation group. The numbers of nerve fibers in anterior vaginal wall of rats in BPNB group, was obviously decreased in 3 days after the operation, reached a minimum value at 1 weeks, and lasting till 3 months. QRT-PCR indicated that the expression of UCHL mRNA in the BPNB group was significantly decreased after 1 week, 1 month and 3 months, while the expression of Nestin was significantly decreased 1 month and 3 months after the operation. Conclusions: Bilateral pudendal nerve block could be used to make rat models of anterior vaginal nerve injury for further exploratory research on pelvic nerve injury theory of pelvic floor dysfunction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tecido Nervoso Limite: Animals Idioma: Zh Revista: Zhonghua Yi Xue Za Zhi Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tecido Nervoso Limite: Animals Idioma: Zh Revista: Zhonghua Yi Xue Za Zhi Ano de publicação: 2019 Tipo de documento: Article