Transcriptome analysis of activated sludge microbiomes reveals an unexpected role of minority nitrifiers in carbon metabolism.
Commun Biol
; 2: 179, 2019.
Article
em En
| MEDLINE
| ID: mdl-31098412
Although metagenomics researches have illuminated microbial diversity in numerous biospheres, understanding individual microbial functions is yet difficult due to the complexity of ecosystems. To address this issue, we applied a metagenome-independent, de novo assembly-based metatranscriptomics to a complex microbiome, activated sludge, which has been used for wastewater treatment for over a century. Even though two bioreactors were operated under the same conditions, their performances differed from each other with unknown causes. Metatranscriptome profiles in high- and low-performance reactors demonstrated that denitrifiers contributed to the anaerobic degradation of heavy oil; however, no marked difference in the gene expression was found. Instead, gene expression-based nitrification activities that fueled the denitrifiers by providing the respiratory substrate were notably high in the high-performance reactor only. Nitrifiers-small minorities with relative abundances of <0.25%-governed the heavy-oil degradation performances of the reactors, unveiling an unexpected linkage of carbon- and nitrogen-metabolisms of the complex microbiome.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
12_ODS3_hazardous_contamination
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2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Esgotos
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Carbono
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Nitrificação
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Microbiota
Idioma:
En
Revista:
Commun Biol
Ano de publicação:
2019
Tipo de documento:
Article